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	Provedor de dados BJM (2) Autor Baldani,José Ivo (2) Gitahy,Patrícia de Medeiros (2) Arrigoni,Enrico de Beni (1) Monnerat,Rose Gomes (1) Salles,Joana Falcão (1) Skøt,Leif (1) Souza,Marlene Teixeira de (1) Mais... Palavra-chave Bacillus thuringiensis kurstaki (1) Biological control (1) Cry gene (1) Cry toxins (1) Diatraea saccharalis (1) Diazotrophic (1) Endophytic bacteria (1) Gluconacetobacter diazotrophicus (1) Herbaspirillum seropedicae (1) Spherical crystal (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2007 (1) 2000 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A Brazilian Bacillus thuringiensis strain highly active to sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae) BJM Gitahy,Patrícia de Medeiros; Souza,Marlene Teixeira de; Monnerat,Rose Gomes; Arrigoni,Enrico de Beni; Baldani,José Ivo. The control of the major sugarcane pest, Diatraea saccharalis, is limited by the stem location of the caterpillar. As part of a long-term project towards the development of an alternative and efficient delivery system of Cry proteins to control the sugarcane borer, the current work describes the selection and characterization of a Brazilian B. thuringiensis strain with prominent activity towards D. saccharalis. Strain S76 was eleven-fold more active than the HD-1 Lepidoptera-standard strain, as estimated by the LC50 of 13.06 µg/L and 143.88 µg/L, respectively. We observed bipiramidal and cuboidal crystals similar to those found in other B. thuringiensis strains with entomopathogenic activity against Lepidoptera and Diptera. In addition, smaller and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Bacillus thuringiensis kurstaki; Cry toxins; Spherical crystal; Diatraea saccharalis. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300028 Use of endophytic diazotrophic bacteria as a vector to express the cry3A gene from Bacillus thuringiensis BJM Salles,Joana Falcão; Gitahy,Patrícia de Medeiros; Skøt,Leif; Baldani,José Ivo. The goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from Bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. The endophytic nitrogen-fixing bacteria Gluconacetobacter diazotrophicus strain BR11281 and Herbaspirillum seropedicae strain BR11335 were used as models. The cry3A gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid N-free medium. The presence of the cry gene was detected by Southern-blot using an internal fragment of 1.0 kb as a probe. The production of delta-endotoxin by the recombinant H. seropedicae strain was detected by dot blot while for G.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gluconacetobacter diazotrophicus; Herbaspirillum seropedicae; Endophytic bacteria; Diazotrophic; Cry gene. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300001 Registros recuperados: 2 Primeira ... 1 ... Última

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